Comparative study onischemia-reperfusion injuries caused by heterogeneity of different positions of biliarysystem in liver graft in rats

Abstract

：Objective Tocomparatively study the ischemia-reperfusion injuries caused by heterogeneity of differentpositions of the biliary system and different construction patterns of the peribiliaryvascular plexus. Methods Thirty rats were randomly divided into 3 groups: Group Ⅰ , sham operated; Group Ⅱ, 1h ischemia inbiliary tract followed by 1h reperfusion; Group Ⅲ, 1h ischemia in biliarytract followed by 2h reperfusion. TUNEL assay, pathomorphology score determination andultrastructural quantitative analysis were performed on epithelium of the hilar bile duct,proximal common bile duct and interlobular bile duct. Results In groupⅡ , TUNEL assay and pathomorphology score showed no statisticaldifference between proximal common bile duct and interlobular bile duct (P＞0.05)but showed significant differences in the hilar bile duct(P＜0.05). Mean volume (V) ofmitochondria and area density of microvilli were obviously serious in the hilar bile ductbut obviously slight in the proximal common bile duct(P＜0. 05). In group Ⅲ,the results of the above detections showed that the most severe was in hilar bile duct,followed by the interlobular bile duct and proximal common bile duct(P＜0. 05). Conclusion Differentinjuries in various parts of the biliary system are caused by heterogeneity of biliaryepithelial cells and construction patterns of the peribiliary vascular plexus. It alsoprovides the experimental basis to explain the higher incidences of hilar bile ductstricture. It could be taken as the best position when the bile duct is anastomosed. Key words: Liver transplantation; Biliary tract; Ischemia-reperfusioninjury; Peribiliary vascular plexus; Rat