Comparative Study on Three Different Systems of Chemiluminescence Flow‐Injection Determination of Leucogen

Abstract

The effects of three systems on the chemiluminescence (CL) intensity have been studied in this paper, such as leucogen–potassium permanganate–rhodamine B, leucogen–cerium (IV)–rhodamine B, and leucogen–luminol–hydrogen peroxide (called system 1, system 2, and system 3, respectively). The mechanism of these reactions is also discussed. Surfactant (CTMAB) has a remarkably sensitive effect on these systems mentioned above. Therefore, three new flow injection chemiluminescence methods for the determination of leucogen have been established. For system 1, the linear range is 8.0×10−8 to 4.0×10−5 g mL−1, with limits of detection 2×10−8 g mL−1; the relative standard deviation is 2.5% (n=11, Cs=4.0×10−6 g mL−1). For system 2, the linear range is 1.0×10−8 to 5.0×10−6 g mL−1, with limits of detection 3×10−9 g mL−1; the relative standard deviation is 5.1% (n=11, Cs=1.0×10−6 g mL−1). For system 3, the linear range is 4.0×10−8 to 2.0×10−6 g mL−1, with limits of detection 1×10−8 g mL−1; the relative standard deviation is 1.3% (n=11, Cs=1.0×10−7 g mL−1). Compared with the three methods above, system 3 is confirmed as the best method. This method has been applied to the determination of leucogen with satisfactory results.