Comparative study on the stability of soybean (Glycine max) β-conglycinin in vivo

Abstract

The immunoreactivity and structural variation of β-conglycinin in digesta from digestive tracts of pigs were measured by inhibition ELISA and sodium dodecyl sulphate polyacrylamide gel electrophoresis, respectively. Results showed that the immunoreactivity disappearance proportion of β-conglycinin significantly increased from stomach to the caecum in all groups (P<0.05). In the stomach, upper-jejunum and middle-jejunum, the immunoreactivity disappearance proportion of β-conglycinin significantly increased among these three groups (P<0.05), while it has no significant difference in ileum and caecum (P>0.05). The α′, α subunits of β-conglycinin were easier to digest than the β subunit. It indicated that immunoreactivity disappearance proportion of β-conglycinin tended to increase with the growth of age and the descending down of digestive tract, while the β subunits of β-conglycinin are more stable to digestion than α′, α subunits.