Abstract
In this work, a comparative study for the fractionation of Trichoderma reesei cellulases on five different hydrophobic interaction chromatography adsorbents (Butyl–Sepharose 4 FF, Phenyl–Sepharose 6 FF, Octyl–Sepharose 4 FF, Epoxy–Sepharose CL-6B and Polypropylene glycol–Sepharose CL-6B) is shown. The influence of the mobile phase composition on the chromatographic behaviour of T. reesei cellulases complex was evaluated using different concentrations of ammonium sulphate in the eluent buffer. A selective separation of β-glucosidase with two-fold increase in specific activity and good recoveries of cellobiase activity were obtained with Butyl–Sepharose 4 FF and Phenyl–Sepharose 6 FF using 7% (w/v) ammonium sulphate in the eluent buffer. A β-glucosidase fractionation was also obtained with Epoxy–Sepharose CL-6B, using 13% (w/v) of the salt in the mobile phase.
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