Abstract
In this paper we report on the results of a comparative study on the performance of Purospher RP 18e, 5 μm, columns and prototypes of monolithic columns named SilicaROD from Merck, Darmstadt, Germany. The studies were performed on HPLC equipment with minimum extra column contribution. The plate height linear velocity dependency of the Purospher RP 18e column showed a minimum of H of about 10–15 μm at a linear velocity of 1 mm/s. The H versus u curves of the monolithic columns followed the same course. Yet, the curves remained flat up to a linear velocity of about 7 mm/s, where the Purospher RP 18e column could not be operated anymore due to the extremely high back-pressure. In conclusion the monolithic HPLC columns showed a column performance equivalent to a column with about 5 μm porous particles and were operated at flow-rates up to 9 ml/min on a conventional HPLC equipment without notable loss in column efficiency. The column back-pressure drop at the monolithic columns was three- to five-times lower than that on the 5-μm particulate column.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
