Abstract
Shuang–Huang–Lian injectable powder (SHL)—a classical purified herbal preparation extracted from Scutellaria baicalensis, Lonicera japonica, and Forsythia suspense—has been used against human adenovirus III (HAdV3) for many years. The combination herb and its major bioactive compounds, including chlorogenic acid, baicalin, and forsythia glycosides A, are effective inhibitors of the virus. However, no comprehensive studies are available on the antiviral effects of SHL against HAdV3. Moreover, it remains unclear whether the mixture of chlorogenic acid, baicalin, and forsythia glycosides A (CBF) has enhanced antiviral activity compared with SHL. Therefore, a comparative study was performed to investigate the combination which is promising for further antiviral drug development. To evaluate their antivirus activity in parallel, the combination ratio and dose of CBF were controlled and consistent with SHL. First, the fingerprint and the ratio of CBF in SHL were determined by high performance liquid chromatography. Then, a plaque reduction assay, reverse transcription polymerase chain reaction (PCR), real-time polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA) were used to explore its therapeutic effects on viral infection and replication, respectively. The results showed that SHL and CBF inhibited dose- and time-dependently HAdV3-induced plaque formation in A549 and HEp-2 cells. SHL was more effective than CBF when supplemented prior to and after viral inoculation. SHL prevented viral attachment, internalization, and replication at high concentration and decreased viral levels within and out of cells at non-toxic concentrations in both cell types. Moreover, the expression of tumor necrosis factor alpha (TNF)-α, interleukin (IL)-1ß, and IL-6 was lower and the expression of interferon (IFN)-γ was higher in both cell types treated with SHL than with CBF. In conclusion, SHL is much more effective and slightly less toxic than CBF.
Highlights
Human adenovirus (HAdV), a nonenveloped DNA virus, is a common causative pathogen of acute respiratory infection
HAdV3 strains of subspecies B1 are the major epidemic strains responsible for severe respiratory disease epidemics and outbreaks worldwide [3,4,5,6,7,8,9,10]
Taken suggest that Shuang–Huang–Lian injectable powder (SHL) can attenuate the inflammatory effects in HAdV3 -stimulated A549 and HEp-2 cells together, these results suggest that SHL can attenuate the inflammatory effects in HAdV3‐stimulated more than CBF
Summary
Human adenovirus (HAdV), a nonenveloped DNA virus, is a common causative pathogen of acute respiratory infection. HAdV infection is more common in childcare and overcrowded conditions, Viruses 2017, 9, 79; doi:10.3390/v9040079 www.mdpi.com/journal/viruses. HAdV species B (serotypes 3, 7, 14, and 55), species C (serotypes 1, 2, 5, and 6), and species E (serotype 4) are the most commonly found in patients with respiratory infection. HAdV3 strains of subspecies B1 are the major epidemic strains responsible for severe respiratory disease epidemics and outbreaks worldwide [3,4,5,6,7,8,9,10]. There is no effective treatment or vaccine against HAdV3 infection and new anti-HAdV3 drugs urgently need to be developed
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.