Abstract
The gel permeation chromatography, combined with laser light scattering and refractive index (GPC-LLS-RI) online method and laser light scattering (LLS) single method, researched the weight-average molecular weight (Mw) of konjac glucomannan (KGM) in this paper. The results show that the determination results of molecular weight of KGM are similar by two method, the molecular weight of KGM is2.476×105 g/mol by online method measured, and it is2.508×105 g/mol by single method measured, compared with only 1.3% difference between the two methods. At the same time, the molecular weight distributions of KGM and conformation index were analyzed by online method, while the second virial coefficient (A2) that characterizes the interaction between KGM molecules and solvent molecules was acquired by single method, which can provide reliable foundation for the molecular chain morphology research of KGM.
Highlights
Konjac glucomannan (KGM) is a water-soluble heteropolysaccharide [1, 2] derived from tubers of the Araceae plant
E gel permeation chromatography, combined with laser light scattering and refractive index (GPC-LLS-RI) online method and laser light scattering (LLS) single method, researched the weight-average molecular weight (Mw) of konjac glucomannan (KGM) in this paper. e results show that the determination results of molecular weight of KGM are similar by two method, the molecular weight of KGM is 2.476 × 105 g/mol by online method measured, and it is 2.508 × 105 g/mol by single method measured, compared with only 1.3% difference between the two methods
The molecular weight distributions of KGM and conformation index were analyzed by online method, while the second virial coefficient (AA2) that characterizes the interaction between KGM molecules and solvent molecules was acquired by single method, which can provide reliable foundation for the molecular chain morphology research of KGM
Summary
Konjac glucomannan (KGM) is a water-soluble heteropolysaccharide [1, 2] derived from tubers of the Araceae plant It is composed of 1,4-linked ββ-D-mannopyranose and ββ-Dglucopyranose units in a molar ratio of 1.6 : 1 with a low degree of acetyl groups at the side chain C-6 position and having a molecular weight on average of 0.67–1.9 million [3]. Gel permeation chromatography, combined with laser light scattering and refractive index (GPC-LLS-RI) online method and laser light-scattering (LLS) single method, has analysed molecular weight besides weight-average molecular weight (Mw), index of conformation (ββ), and the second coefficient (AA2), etc. Compare molecular weight of KGM utilization of GPC-LLS-RI and LLS by static laser light scattering
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