Abstract
BackgroundMethicillin resistant Staphylococcus aureus (MRSA) is recognized worldwide as a leading cause of hospital and community infections. Biofilm formation by MRSA is an extremely important virulence factor to be understood. Our aim was to establish phenotypic and genotypic characterization of virulence factors among 43 MRSA clinical isolates in a Tunisian hospital.MethodsWe investigated enzymatic profiles, biofilm production and prevalences of genes encoding intracellular adhesion molecules (icaA and icaD), Microbial Surface Components Recognizing Adhesive Matrix Molecules genes (fnbA, fnbB and cna) and exoenzymes genes (geh, sspA and sspB).ResultsOur findings revealed that caseinase, gelatinase, lipase and lecithinase activities were detected in 100%, 100%, 76.6% and 93.3% of cases respectively. This study showed that 23 strains (76.7%) were slime producers on Congo red medium. Furthermore, 46.5% and 53.5% of isolates were respectively highly and moderately biofilm-forming on polystyrene. Significant association was found between both biofilm tests. PCR detection showed that 74.4%, 18.6%, 69.8%, 65.1% and 74.4% of isolates harbored fnbA, fnbB, icaA, icaD and cna genes respectively. In addition, 34.9%, 18.6% and 30.2% of MRSA strains were found positive for sspA, sspB and geh genes respectively. Further, statistical data showed that the presence of the fnbA and fnbB genes was significantly associated with a high biofilm production on polystyrene. However, no statistical association was observed for the icaA, icaD and cna genes.ConclusionsThis study indicates that the detection of fnbA and fnbB contributing to the first step of biofilm formation has been predictable of high biofilm production. As studied factors contribute to MRSA virulence, this research could be of value in orienting towards the development of new preventive and therapeutic measures.
Highlights
Methicillin resistant Staphylococcus aureus (MRSA) is recognized worldwide as an important bacterial pathogen causing a wide range of infections ranging from skin and soft tissue lesions to lethal infections
Our aim was to establish a phenotypic characterization of virulence factors among MRSA clinical isolates in a Tunisian Hospital, to determine prevalences of genes encoding intracellular adhesion molecules, Haddad et al BMC Infectious Diseases (2018) 18:560
Distribution of isolates A total of 43 MRSA strains were collected
Summary
Methicillin resistant Staphylococcus aureus (MRSA) is recognized worldwide as an important bacterial pathogen causing a wide range of infections ranging from skin and soft tissue lesions to lethal infections (osteomyelitis, endocarditis, pneumonia and septicaemia). It is regarded as a major world health threat with a substantial increase in morbidity and mortality [1]. Our aim was to establish a phenotypic characterization of virulence factors among MRSA clinical isolates in a Tunisian Hospital, to determine prevalences of genes encoding intracellular adhesion molecules (icaA and icaD), Haddad et al BMC Infectious Diseases (2018) 18:560. Our aim was to establish phenotypic and genotypic characterization of virulence factors among 43 MRSA clinical isolates in a Tunisian hospital
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