Abstract
Abstract Bhavprakash described the Abhava dravyas for group of drugs like Jeevaniya gana, Ashtavarga etc which were not available that time also, now a days number of valuable herbs are extincted due to lack of propagation methods. Safed musali is one such drug which is used in large quantities by Ayurvedic, Unani and other traditional practitioners, now it is rare. With the help of biotechnology presently we can propagate the endangered species like safed musali in some artificial conditions and cultivate them in the natural habitat. Tissue culture technology is one of the effective bio-technological tool to propagate large number of sampling which have exact genetic makeup of the mother plant, this is possible as each and every cell of a plant is totipotent. Tissue culture does not much effect or alter the natural characterstics of safed musali but increases the yield. Keywords : Safed musali, tissue culture, bio-technology, totipotent.
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