Abstract
Objective: The present study aims at the investigation of antioxidant activities both by qualitative (thin-layer chromatography [TLC] bioautography) and quantitative (in vitro) methods and thereby comparison of the same in different parts of the plants of Clitoria ternatea (Fabaceae). Methods: Leaves, stems, and seeds were macerated with methanol separately and preliminary phytochemical screening was carried out. The extracts were developed using two mobile phase combinations such as Toluene: Ethyl acetate: Formic acid (5:4:0.2) for test 1 and toluene: Ethyl acetate: Methanol (5:3:2) for test 2 on silica gel GF254 TLC plates and further sprayed with DPPH. Antioxidant and free radical scavenging activities were assayed by inhibition of lipid peroxidation and DPPH method with the different concentrations of test extracts (25, 50, 100, 150 and 200 μg/ml) and standard ascorbic acid. Results: The methanolic extract of the leaves, stems, and seeds revealed to possess chiefly alkaloids, phenols, and flavonoids and have shown promising antioxidant activity by all the methods in dose-dependent manner. Leaves were found to be most potent having IC50 value of 111.652 µg/ml and 106.683 µg/ml which is almost similar to the stems (IC50 of 111.685 µg/ml and 106.26 µg/ml), respectively, in DPPH method and inhibition of lipid peroxidation. Seeds failed to produce any significant activity in any of the methods performed. Conclusion: The methanolic extract of leaves and stems can act as a potential antioxidant and antiradical, which are highly comparable with standard.
Highlights
Clitoria ternatea (Fabaceae) is a perennial herb which is commonly known as Aparajita [8]
Thin-layer chromatography bioautography study of antioxidant activity The thin-layer chromatography (TLC) chromatogram shows some major bands at 366 nm
Most of the bands turned into yellow color when the plate was sprayed with DPPH (0.2% in methanol), which indicates the presence of antioxidant substances
Summary
Reactive oxygen species initiate the oxidation process and affect the metabolism of organisms Overproduction of these free radicals leads to severe pathological conditions [1] and damage of cellular components by covalent binding and lipid peroxidation leading to tissue injury and in turn to the development and/or progression of various conditions or diseases such as aging, carcinogenesis, physical injury, infection and cardio vascular, liver cirrhosis, diabetes, nephrotoxicity, and degenerative diseases [2,3]. The brown or black in colored seeds are 4.5–7 mm long and 3–4 mm wide [9]. The seeds have purgative, cathartic activity and useful in visceralgia [10]
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