Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane.

Suryani Saallah,Sharinee Saallah,Siti Mazlina Mustapa Kamal,Wolyna Pindi,Jumardi Roslan,Umi Hartina Mohamad Razali,Flavian Sheryl Julius,Mohd Rosni Sulaiman,Khairul Faizal Pa’Ee

doi:10.3390/molecules26092564

Abstract

Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.

Highlights

The collagen obtained were denoted as D-Pepsin-Solubilized Collagen (PSC) and UF-PSC, respectively
Was significantly (p < 0.05) higher than for D-PSC (5.15 %), which indicated that separation of collagen using the UF membrane is more efficient compared with the conventional method
UF-PSC exhibits a higher collagen yield (11.39%) than D-PSC (5.15%), indicating the efficiency of the process to recover collagen

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Sea cucumbers are marine animals that belong to the phylum Echinodermata from the class Holothuridea. They have soft, elongated bodies with leathery skin, resembling a cucumber. About 1250 species of sea cucumber have been identified, in the benthic and deep seas across the world. Holothuria is the most important sea cucumber species with high economic and trade values. Most of Holothuria species can be found in South-East Asian countries, including Malaysia, with the most harvested species being Holothuris scabra (sandfish), Holothuria nobilis (black teatfish), and Holothuris fuscogilva (white teatfish) [1,2]

Methods

Results

Conclusion