Abstract
Abstract A series of lipidic amino acids and peptides were investigated by systematic change of the mobile phase composition using traditional octadecylsilica stationary phase and the newly developed Supelcosil™ LC-ABZ column. The mobile phases contained various concentrations of methanol and acetonitrile combined with 0.1% trifluoroacetic acid (TFA). The log k′ values were plotted as a function of organic phase concentration in the mobile phase. In some cases parabolic curves were obtained on the traditional octadecyl silica stationary phase due to the presence of the free silanol groups, while on the Supelcosil™ LC-ABZ column straight lines were obtained. Much lower concentration of organic phase was needed for getting similar retention times on the Supelcosil™ LC-ABZ column, so no retention was observed at higher organic phase concentrations where the silanol effect could have been observed. The slope and the intercept values of the linear section of the log k′ vs organic phase concentration lines were...
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