Comparative Study of the Lipophilicity of Selected Tauro-Conjugates Bile Acids Determined with the Use of RPTLC and RPHPTLC Methods

Abstract

Lipophilicity of selected tauro-conjugates bile acids such as: taurocholic acid (TC), taurodeoxycholic acid (TDC), taurochenodeoxycholic acid (TCDC), and taurolithocholic acid (TLC), by means of RPTLC and RPHPTLC methods was found. Reversed–phase thin layer chromatography was performed on RP18F254, RP18WF254, and on RP2F254 chromatographic plates with the use of a mixture of an organic modifier (methanol, acetone, dioxane)–water in different volume compositions. The obtained chromatographic parameters of lipophilicity (RMW and ϕ0) indicates that regardless of applied chromatographic conditions, the lipophilicity of four examined tauro-conjugates bile acids should decrease in the following order: TLC > TCDC ≅ TDC > TC. A good correspondence between experimentally determined lipophilic parameters and the theoretical value of lipophilicity (log Pvirtual) for investigated bile acids was observed. The cluster analysis (CA) of lipophilic parameters indicates a large similarity of lipophilicity for tauro-conjugates bile acids. All examined bile acids form exactly one cluster in almost all chromatographic systems used.