Comparative study of the effects of MCPA, butylate, atrazine, and cyanazine on Selenastrum capricornutum

Abstract

The herbicides MCPA, butylate, atrazine and cyanazine are extensively used in Canadian agriculture and information regarding their effects on indigenous biota is scarce. Phytotoxicity assessments were conducted in the laboratory on the common green alga Selenastrum capricornutum using both the active ingredient of the herbicides and their formulated products (for MCPA and butylate). Endpoints determined after the 96 h exposure included algal population growth inhibition (IC 50—cell counts), percent lethality (LC 50—flow cytometry derived) and photosynthetic electron transport inhibition (EC 50—fluorescence induction). Pesticide formulations had greater toxic effects than the active ingredient alone. The 96 h IC 50 (50% Inhibition Concentration) and LOEC (Lowest Observable Effects Concentration) using cell counts of S. capricornutum were 18.4 and 8.9 mg l −1 respectively for MCPA-active ingredient and for MCPA-formulated, these were 0.62 and 0.0062 mg l −1 respectively. Those for butylateactive ingredient were 61.0 and 8.3 mg l −1 and for butylate-formulated 1.46 and 0.17 mg l −1, respectively. The triazines active ingredient, which are photosynthetic inhibitors, had greater effects than either the MCPA or butylate. The IC 50 for cyanazine and atrazine were 0.059 and 0.026 mg l −1, respectively. By comparing the IC 50 and LC 50 values for the tested active ingredients, it was found that the effects of atrazine were algicidal, whereas those of cyanazine, butylate and MCPA were algistatic.