Comparative study of the effect of Omega-3 and the nano-loaded Propylthiouracil on thyroxine-induced hyperthyroidism in male white rats

Abstract

This study was conducted to find out the therapeutic effect of Omega-3 and the zinc oxide nano-loaded propylthiouracil drug in the treatment of thyroxine-induced hyperthyroidism. The results show a significant increased (p<0.05) in serum of Triiodothyronine(T3) and Thyroxine (T4) hormones with a decreased Thyroid stimulating hormone (TSH) level in T1 group compared with the control group. On the other hand, the results showed a significant decrease (P < 0.05) in (T3) and (T4) with increased (TSH) levels in (T2, T3) groups compared to (T1) group. There was a significant (p < 0.05) increase in the level of Malondialdehyde (MDA)with a decrease in the level of antioxidants Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT) in the (T1) group in comparison with the control group. Also, the results showed a significant (p < 0.05) decrease in the level of MDA with an increase in the level of antioxidants (GSH, SOD, CAT) in the (T2, T3) groups as compared to the (T1) group. The results of the current study showed that there was a significant increase (P < 0.05) in the level of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) in the (T1) group as compared to (C) group, also, these results showed a significant (p < 0.05) decrease in the level of ALP, ALT, AST in the (T2, T3) groups compared to (T1) group. Several methods were used in the examination of the drug propylthiouracil nano-loaded on zinc oxide, as they included the following tests on the Fourier transform infrared Spectroscopy (FT – IR) and X-ray diffraction X-ray diffraction spectrum (XRD) as well as the use of atomic force microscope Atomic Force Microscope (AFM), the process of intercalation of the drug between layers of zinc oxide was carried out by the direct ion exchange method. This technique showed that propylthiouracil gave spectra indicating the success of the intercalation process, which was used in the subsequent stages of the study