Abstract
Schisandra chinensis Bail. polysaccharides from China (CSP) and Russia (RSP) were separated by DEAE-52 cellulose column chromatography. The content of neutral polysaccharide was determined by the phenol concentrated sulfuric acid method, the content of acid polysaccharide by the hydroxybiphenyl method, and the monosaccharide composition and molecular weight arrangement of CSP and RSP by 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization HPLC. The effects of CSP and RSP on the exercise endurance of mice were compared by the forelimb grip strength test, rota-rod test and weight-bearing swimming. The results showed that one neutral polysaccharide and three acidic polysaccharides could be eluted from a DEAE-52 cellulose column from CSP and RSP, respectively. The content of acidic and neutral polysaccharides in RSP was higher than that in CSP, and the anti-fatigue effect of RSP was more significant than that of CSP.
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