Abstract
High-performance liquid chromatographic-tandem mass spectrometric (HPLC-MS-MS) methods were developed for the analysis of 13 bioactive constituents of Zizyphi Spinosae Semen ( ZSS), and for samples of ZSS fried at different temperatures and from different production areas. The results showed that the content of bioactive constituents increased with the increase in frying time from 65 to 80 min at 150 °C; among the 14 samples of ZSS from different production areas, those from Shanxi Changzhi, Shandong Jining, and Hebei Xingtai had a higher content of bioactive constituents. This analytical method can accurately and quickly analyze the bioactive constituents of ZSS and provide a scientific basis for its quality control.
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