Abstract
Sida rhombifolia or sidaguri, was taken from two regions in East Java Province, Indonesia, namely Batu (SB) and Ngawi (SN), was investigated in this study. The two samples were determined the content of secondary metabolites by using LC-MS/MS. They were also evaluated for their phytochemical content, total phenolic content (TPC), and antioxidants activity, using the DPPH and ABTS methods. The highest TPC (126.93 ± 0.63 mg GAE / g) was observed in the ethyl acetate fraction of SN. The antioxidant activity against DPPH and ABTS from crude extracts and fractions showed that SB was more potent than SN, although the values were both less active. A total of 54 secondary metabolites were identified by the LC-MS-MS method from both species, with SB samples producing more secondary metabolites (48 compounds) than SN (37 compounds). These compounds are included in lipids, alkaloids, ester lactones, benzoic acid derivatives, steroids, carboxylic acid derivatives, flavonoids, and phenyl propanoic.
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