Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules

D Hoang Vu,T Giang Do

doi:10.4103/0975-1483.63168

Abstract

Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.

Highlights

Amoxicillin, formerly amoxycillin [Figure 1 a], is a moderatespectrum beta-lactam antibiotic used to treat infections caused by penicillin-sensitive gram-positive bacteria as well as some gram-negative bacteria.[1]
To overcome its sensitivity to destruction by beta-lactamases, amoxicillin has been co-administered with clavulanic acid, a potent betalactamase inhibitor[2] in pharmaceutical preparations
This antibiotic is incompletely absorbed from the gastrointestinal tract, and absorption is reduced by the presence of food in the stomach

Summary

Introduction

Amoxicillin, formerly amoxycillin [Figure 1 a], is a moderatespectrum beta-lactam antibiotic used to treat infections caused by penicillin-sensitive gram-positive bacteria as well as some gram-negative bacteria.[1] Amoxicillin is resistant to inactivation by gastric acid. It is usually the drug of choice because it is more rapidly and more completely absorbed than other beta-lactam antibiotics when orally administered. To overcome its sensitivity to destruction by beta-lactamases, amoxicillin has been co-administered with clavulanic acid, a potent betalactamase inhibitor[2] in pharmaceutical preparations. To produce a wider spectrum of activity, Cloxacillin is a semisynthetic penicillin used as the sodium salt [Figure 1 b] to treat staphylococcal infections due to

Methods

Results

Conclusion