Comparative study of RNA metabolism in freshly isolated protoplasts and callus cultures of Parthenocissus tricuspidata crown gall

Abstract

AbstractRNA metabolism was compared in protoplasts and cells of Parthenocissus tricuspidata crown gall callus. A rapid increase in the permeability to precursors during the first three hours following the termination of protoplast isolation was observed. Consequently, RNA synthesis occurred at a higher rate in protoplasts than in whole cells. On the other hand, protoplasts and callus tissues showed similar kinetics of incorporation of precursors into mature rRNA's. Pulse‐chase experiments showed the maturation rate to be nearly the same in both cases.