Abstract
Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.
Highlights
Escherichia coli O157:H7 was first recognized as a human pathogen following two hemorrhagic colitis outbreaks in 1982
A. hydrophila was growing in Ampicilin starch agar phenol red, S. enterica was grown in Bismuth sulfite agar, S. aureus was grown in Staphylococcus selective Agar (CM 310) and E. oliO157:H7 was grown in MacConkey agar obtained from Beijing Land Bridge Technology co., LTD, China
S. aureus has been cited as an example for grampositive bacteria and other bacteria as an example for gramnegative
Summary
Escherichia coli O157:H7 (designated by its somatic, O, and flagellar, H, antigens) was first recognized as a human pathogen following two hemorrhagic colitis outbreaks in 1982. Salmonella spp. has been associated with fecal contamination, and it’s a most important cause of human pathogens It causes gastroenteritis and is a leading cause of food related deaths. DNA is a polymer made of nucleotide monomers, Deoxyribionuclic acid (DNA) contain unique genetic information (genetic sequences information) for every human being and livening organism president in the earth, when obtaining DNA sequences data, small errors in this information could results in large mistake in identification. It most commonly exists as a double-stranded macromolecule where two polynucleotide strands are held together by hydrogen bonds between the complementary nitrogenous bases. The efficiency of a high quality DNA of each one to obtain highly extract DNA from real samples to choose the suitable extraction methods should be applied to PCR to use the target DNA in further experiments like DGGE, RT-PCR, electrochemical biosensor and etc
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
