Comparative study of modified quantitative buffy coat and two rapid tests in comparison with peripheral blood smear in malaria diagnosis in mumbai, India.

Manali M Kocharekar,Debjani Dasgupta,Sougat S Sarkar

doi:10.1155/2014/194651

Manali M Kocharekar, Debjani Dasgupta + Show 1 more

Open Access

https://doi.org/10.1155/2014/194651

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Abstract

In order to identify a quick and reliable technique for accurate diagnosis of malaria, study of the efficiency of the tests such as Parahit total (HRPII & aldolase Ag), Advantage mal card (parasite specific LDH), and modified QBC was done in comparison with conventional blood smear microscopy. One hundred patients infected with P. vivax and 101 infected with P. falciparum were included in this study. The sensitivity of Parahit total, Advantage mal card, and modified QBC for P. falciparum detection was 70.3, 95%, and 98%, and specificity was 98%, 98%, and 96%, respectively. The sensitivity of Parahit total, Advantage mal card, and modified QBC for P. vivax detection was 73%, 97.0%, and 98%, respectively, and specificity of all the tests was 98%. On day 15, in falciparum arm, Advantage mal card and Parahit total showed 8 (7.92%) and 59 (58.41%) false positives. On day 15, in vivax arm, Parahit total revealed 52% false positives. The study indicated that modified QBC could be only used where appropriate facilities are available. Advantage mal card was a better follow-up tool than Parahit total.

Highlights

The National Vector Borne Disease Control Programme (NVBDCP) of India reports about 2 million malaria parasite positive cases annually, of which about 50% are Plasmodium falciparum [1].The gold standard for malaria detection is microscopic detection which is tedious and dependent on technical expertise
A total of 1301 patients who were suspected with malaria were screened for malarial parasites by thick and thin Peripheral Blood Smears (PBS) from May 2010 to November 2011
Out of the 50 negative patients, one false positive was detected as compared with PBS microscopy

Summary

Introduction

The National Vector Borne Disease Control Programme (NVBDCP) of India reports about 2 million malaria parasite positive cases annually, of which about 50% are Plasmodium falciparum [1]. The gold standard for malaria detection is microscopic detection which is tedious and dependent on technical expertise. Tests like Parahit total, Advantage mal card,and QBC have been introduced. In view of the seriousness of the malarial infection and paucity in current availability of diagnostic facilities across India, we have conducted a comparative study of the above tests with microscopy at the baseline and on eight and 15 days of followup

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