Abstract
Several therapeutic products based on mesenchymal stem cells (MSCs) have been translated into clinical applications. MSCs should undergo in vitro culture before a sufficient quantity can be achieved. Hence, both expansion kinetics and the biological characteristics of derived cells from primary culture are pertinent to their applications. In the present study, MSCs were isolated from rat bone marrow and adipose tissue (designated as bMSCs and aMSCs, respectively) and cells were comparatively analyzed regarding cell morphology, proliferation, colony formation, differentiation potential, and immunophenotype following the long-term subculture. No apparent differences could be noticed concerning the morphology between bMSCs and aMSCs. The long-term subculture made both types of cells smaller, weakened their colony-forming ability, and stimulated the proliferation rate. However, bMSCs demonstrated better proliferation and colony-forming ability than aMSCs. No significant difference was observed about the expression of some immunophenotypes (i.e. CD29+/CD90+/CD34-/CD45-) regardless of cell types or population doublings. Notably, bMSCs, but not aMSCs, maintained the differentiation potential well after the long-term subculture. The present study demonstrates that MSCs derived from different tissues can be well expanded for the long term, although cells display gradually declined self-renewal and differentiation potentials to different extents depending on the tissue origins.
Highlights
Regenerative medicine is a growing field that aims to treat currently unmet clinical indications such as diabetes, cardiovascular disease, and neurological disorders by restoring or maintaining tissue function (Heathman et al, 2015)
For cells derived from adipose tissue, far fewer cells were present on day 2 and some spindle-shaped cells appeared on day 5 (Figure 2A, yellow arrow)
In the present study, bone morrow-derived MSCs (bMSCs) and adipose tissuederived MSCs (aMSCs) were isolated from rats and subjected to adherent subculturing
Summary
Regenerative medicine is a growing field that aims to treat currently unmet clinical indications such as diabetes, cardiovascular disease, and neurological disorders by restoring or maintaining tissue function (Heathman et al, 2015). Mesenchymal stem cells (MSCs) have tremendous potential for applications in regenerative medicine due to the abundant availability and potentials of self-renewal and differentiation (Lim et al, 2016). Since Pittenger et al demonstrated that human bone marrow-derived MSCs could be successfully induced to undergo multilineage differentiation in 1999 (Pittenger et al, 1999), thousands of studies have been carried out with the objective of translating MSCs in clinical settings. It was reported that MSCs that survived in vivo had the characteristics of pericytes and could maintained vasomotion, vascular maturation, and regulation of extracellular matrix turnover through mechanisms similar to the signal transduction between paracrine and other tissues (Dijk et al, 2015). According to the latest update at ClinicalTrials.gov, there were
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