Abstract
Abstract 1. 1. Immunological and biochemical properties of skeletal muscle tropomyosin were investigated to elucidate phylogenetic relationships among four extant species of horseshoe crabs, Tachypleus tridentatus, T. gigas, Carcinoscorpius rotundicauda , and Limulus polyphemus . 2. 2. Immunodiffusion tests using an antiserum against tropomyosin from T. tridentatus showed no difference of antigenicities among the four species. Peptide patterns of enzymatically or chemically cleaved tropomyosin also represented no significant difference. 3. 3. However, electrophoretic mobilities of the proteins were found to be significantly different between L. polyphemus and three other species when long distance electrophoresis was conducted. 4. 4. The mobility difference revealed a distinctive property of L. polyphemus tropomyosin and confirmed that the species is phylogenetically differentiated far from the other species.
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