Comparative Study of Heavy Metal Uptake and Analysis of Plant Growth Promotion Potential of Multiple Heavy Metal-Resistant Bacteria Isolated From Arable Land.

Abstract

Heavy metal-induced pollution is a serious environmental concern. This study was aimed at exploring indigenous heavy metal-resistant and plant growth promoting bacteria from arable land that might be useful for developing green strategies to counter the challenges related to bioremediation and sustainable agriculture. A thorough screening and characterization of all the twenty heavy metal-resistant bacterial isolates obtained in this study was done. Of these, three potent isolates were further analyzed to unravel their heavy metal resistance and uptake potentiality. Minimum inhibitory concentration determination depicted considerable tolerance (≥ 500µg/mL) of the three isolates to Ni, Zn, Fe, Cd, Cu, etc. Growth kinetics of the isolates in presence of various heavy metals indicated differences between normal and metal-induced growth. pH tolerance and pigmentation ability of the isolates were also analyzed. Inductively Coupled Plasma-Mass Spectrometry study revealed maximum Cd uptake by the isolates during exponential phase of growth. One of the isolates demonstrated plant growth promotion ability detected using different in vitro qualitative screening tests. Molecular identification using 16S rRNA depicted the isolates as strains of Pseudomonas aeruginosa. This was the first study of heavy metal-resistant and plant growth promoting bacteria from this region. Further exploration of such multi metal-resistant indigenous bacteria may pave the way for designing effective strategies for bioremediation and sustainable agriculture.