Comparative study of gut microbiota from decomposer fauna in household composter using metataxonomic approach.

Abstract

Household composter is one of the fragmented habitats, which still ensures the survival of many animals, especially decomposer fauna. The components of organic matter in the composter are complex, thus providing opportunities for obtaining high diversity of decomposer fauna. The ability of decomposer fauna to break down organic matter may be supported by their gut microbiota. In this study, we compared the diversity, the abundance and distribution of gut prokaryotic microbiota among several decomposer fauna members, namely cockroaches, millipedes, beetle larvae, and snails, as well as the compost from a household composter in Surabaya, Indonesia, using a metataxonomic approach. Microbial community DNA was isolated from the guts of four types of dominant decomposer fauna and the compost. A total of 42 phyla and 497 genera were observed in five samples with the predominant bacteria belonging to the Proteobacteria phylum which appeared in all samples. The highest diversity of gut bacteria was found in cockroach although not higher than the compost as a habitat for the decomposer fauna. A total of 1131 operational taxonomic units derived from 232 genera were found in compost and guts of four decomposer fauna species. Similarities between the microbial community structures found in the gut of the four decomposer fauna to those found in compost indicated that the environment had a strong effect on the overall gut microbiota of the decomposer fauna.