Abstract
Investigation to ascertain the levels of glutathione S-transferase (GST) activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS) obtained from apparently healthy and clinically confirmedmalarious subjects/volunteers was carried out. The incubation of human erythrocytes with 1-chloro-2,4- dinitrobenzene (CDNB) resulted in almost quantitative conjugation of glutathione (GSH) to form S-(2,4-dinitrophenyl) glutathione. The reaction formed the basis for the spectrophotometric determination of GST activity. The levels of GST activity of the red cell genotypes was in the order of bAA0.05) affect erythrocyte GST activity of non-malarious subjects/volunteers. The study suggests that GST activity evaluation might be a reliable biochemical marker and possess promising rational for diagnostic potential in malaria
Highlights
Blood of HbSS genotype and malarious blood samples were from patients attending clinics at the Federal Medical Center (FMC), Imo State University
The erythrocyte haemolysate was used for the determination of glutathione S-transferase (GST) activity
The mean (+S.D) activity of glutathione S-transferase (GST) of three erythrocyte genotypes (HbAA, HbAS and HbSS) of blood samples obtained from nonmalarious and malarious human subjects/volunteers is presented in Table 1 below
Summary
Selection of Volunteers/ Experimental Design: Non-Malarious Subjects/Volunteers (Control Group): Seventy (70) male (61-73kg) subjects/volunteers of confirmed HbAA (25), HbAS (25) and HbSS (20) genotypes between the ages of 20-28years enrolled for this study. The female category was made up of forty-two (42) subjects/volunteers (55-69kg); HbAA (20), HbAS (20) and HbSS (12). Port Harcourt, Nigeria, granted approval for this study and all subjects/ volunteers involved signed an informed consent form. This conducted study was in accordance with the ethical principles that have their origins in the Declaration of Helsinki. Blood of HbSS genotype and malarious blood samples were from patients attending clinics at the Federal Medical Center (FMC), Imo State University. These centers are located in Owerri, Imo State, Nigeria. The erythrocyte haemolysate was used for the determination of GST activity
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Applied Sciences and Environmental Management
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
