Abstract
Marbling is one of the important factors among the characteristics influencing meat quality. Among the candidate genes, MYBPC1 and CDC10 genes are considered as functional genes, responsible for marbling. The present study was conducted to investigate the existence of polymorphism and domain variants in the upstream regulatory region of CDC10 and MYBPC1 genes in Iranian fat-tailed (Lori-Bakhtiari) and nonfat-tailed (Zel) sheep breeds. Blood samples were taken randomly from 300 sheep (150 samples per each breed). DNA extraction was performed by modified salting-out method. Fragments with the length of 291 and 372bp from the upstream regulatory region of MYBPC1 and CDC10 genes were amplified by specific primers and different allelic forms in both marker sites were detected by single-strand conformation polymorphism (SSCP) method. In order to verify observed banding patterns (genotypes) and also to investigate the presence of mutations in the sequence, one sample from each banding pattern was sequenced. MYBPC1 marker site showed monomorphic pattern, but the CDC10 was polymorph in both breeds. The allele A was the most frequent allele (0.90 and 0.77%) in Zel and Lori-Bakhtiari breeds respectively. Genotype frequencies of AA were the highest (83.33 and 59.71%) compared with that of BB genotypes (2.90 and 5.04%) in both Zel and Lori-Bakhtiari breeds respectively. Comparison between genotype frequencies by Chi-square test showed significant differences (P < 0.01) among the two studied breeds. The results of multiple sequence alignment revealed six single nucleotide polymorphisms (SNP) in the amplified fragment of CDC10 marker site. Further bioinformatics analysis revealed 16 motifs for this marker site. This study was the first effort for appraising these genes as a candidate genes affecting marbling in sheep. Therefore, supplementary research in these two studied breeds, as well as more studies on other components of these genes in respect to other sheep breeds, is recommended.
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