Abstract
C57BL/6N is the most widely used inbred mouse strain applied in a wide variety of research areas including cancer, cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. To compare the fertilization rates of C57BL/6NKorl mice with two commercial C57BL/6N stocks, differences in reproductive organ structures, sperm and egg numbers, fertilization rates, and embryo development rates among C57BL/6NKorl (Korea FDA source), C57BL/6NA (USA source), and C57BL/6NB (Japan source) mice were determined. Among the stocks, no significant differences were detected in organ weight and histological structure of male and female reproductive organs, although body weight was higher in C57BL/6NKorl mice than that in the other groups. The concentration and morphology of sperm and eggs in C57BL/6NKorl mice were similar to those of C57BL/6NA and C57BL/6NB mice. Furthermore, the three stocks had similar in vitro fertilization and embryo development rates, although these rates tended to be higher in C57BL/6NB mice. Pup body weight was higher in C57BL/6NKorl and C57BL/6NB mice than that in C57BL/6NA mice. The results of the present study suggest that C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice obtained from three different sources have similar fertilization and embryo development rates, although there were slight differences in the magnitude of their responses rates.
Highlights
In vitro fertilization (IVF) can be considered a valuable tool for efficient assisted reproduction in genetically engineered (GE) mice and highly valuable animals [1]
Mean body weight of female was slightly higher in C57BL/6NKorl mice than that in C57BL/6NA mice and C57BL/6NB mice, but that of male was significantly higher C57BL/6NKorl mice and C57BL/6NB mice than that in C57BL/6NA mice (Table 1)
We compared the reproductive organ structure, gametes concentration, fertilization rate, and embryo development rate of C57BL/6N mouse stocks derived from three different sources
Summary
In vitro fertilization (IVF) can be considered a valuable tool for efficient assisted reproduction in genetically engineered (GE) mice and highly valuable animals [1]. It may be used for reconstituting mouse strains from cryopreserved sperm and/or oocytes, which enables laboratories to archive valuable GE mouse, thereby reducing animal numbers, rack space, and maintenance breeding [2].
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