Comparative study of environmental pollutants bisphenol A and bisphenol S on sexual differentiation of anteroventral periventricular nucleus and spermatogenesis

Naham John,Waheed Ullah,Sarwat Jahan,Iftikhar Alam,Suhail Razak,Humaira Rehman,Tayyaba Afsar,Mehwish David,Ali Almajwal

doi:10.1186/s12958-019-0491-x

Naham John, Waheed Ullah + Show 7 more

Open Access

https://doi.org/10.1186/s12958-019-0491-x

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Abstract

BackgroundBisphenol A is well known endocrine-disrupting chemical while Bisphenol S was considered a safe alternative. The present study aims to examine the comparative effects of xenobiotic bisphenol-A (BPA) and its substitute bisphenol-S (BPS) on spermatogenesis and development of sexually dimorphic nucleus population of dopaminergic neurons in the anteroventral periventricular nucleus (AVPV) of the hypothalamus in male pups.MethodsSprague Dawley rat’s pups were administered subcutaneously at the neonatal stage from postnatal day PND1 to PND 27. Thirty animals were divided into six experimental groups (6 animals/group). The first group served as control and was provided with normal olive oil. The four groups were treated with 2 μg/kg and 200 μg/kg of BPA and BPS, respectively. The sixth group was given with 50 μg/kg of estradiol dissolved in olive oil as a standard to find the development of dopaminergic tyrosine hydroxylase neurons in AVPV regions. Histological analysis for testicular tissues and immunohistochemistry for brain tissues was performed.ResultsThe results revealed adverse histopathological changes in testis after administration of different doses of BPA and BPS. These degenerative changes were marked by highly significant (p < 0.001) decrease in tubular and luminal diameters of seminiferous tubule and epithelial height among bisphenols treated groups as compared to control. Furthermore, significantly increased (p < 0.001) TH-ir cell bodies in the AVPV region of the brain with 200 μg/kg dose of BPA and BPS was evident.ConclusionIt is concluded that exposure of BPA and BPS during a critical developmental period can structural impairments in testes and affects sexual differentiation of a dimorphic dopaminergic population of AVPV region of hypothalamus in the male brain.

Highlights

Bisphenol A is well known endocrine-disrupting chemical while Bisphenol S was considered a safe alternative
The sixth group was given with 50 μg/kg of estradiol dissolve in olive oil as a standard to a find development of dopaminergic tyrosine hydroxylase neurons in anteroventral periventricular nucleus (AVPV) regions
The current investigation provides the warning of possible toxic effects of exposure of BPA substitute “BPS” during early life period

Summary

Introduction

Bisphenol A is well known endocrine-disrupting chemical while Bisphenol S was considered a safe alternative. Phthalates help make plastic, like pacifiers, flexible. Due to his widespread applications, the use of BPA has gathered cumulative consideration over the last decade, in terms of human safety. Fetal exposure to high doses of dibutyl phthalate was shown to cause a testicular dysgenesis syndrome (TDS)-like phenotype in the rats [2]. TDS is a result of disruption of embryonal programming and gonadal development during fetal life [2] Various reports established that BPA acts as an endocrine disrupting chemical and its exposure can affect the reproductive system of a male by disturbing spermatogenesis and fertility, its exposure during development causes organizational effects on brain [3,4,5,6,7,8,9,10]. It is suggested that BPA’s actions are greater during development[13,14,15]

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