Comparative study of electron transport system activity and oxygen consumption of amphipods from caves and surface habitats

Abstract

Summary1. Respiratory electron transport system (ETS) activity and oxygen consumption were measured at 10 °C for the hypogean amphipods Niphargus stygius (from two epikarst locations) and N. krameri (from a sinking river in a cave) and the epigean amphipod Gammarus fossarum (inhabiting a spring and a river).2. ETS activity and oxygen consumption were compared between caves and the two surface locations, and between hypogean and epigean amphipods. ETS activities were found not to differ between animals from different locations, or between epigean and hypogean amphipods. As expected, the oxygen consumption of N. krameri and N. stygius was lower than that of G. fossarum.3. The high ratio between ETS activity and oxygen consumption (ETS/R ratio) showed that N. krameri and N. stygius possess high metabolic potential that can be exploited for energy recovery and rapid restoration of body reserves immediately following the appearance of favourable conditions (food and/or oxygen). In contrast, G. fossarum exhibited a low ETS/R ratio, indicating exploitation of a great proportion of the metabolic potential for standard metabolism.4. Feeding in N. stygius increased oxygen consumption but not ETS activity, and thus fed N. stygius had a much lower ETS/R ratio than starved ones.5. A relatively high metabolic potential (i.e. high ETS/R ratio) may be an adaptation to hypogean life in amphipods, in that it improves survival under poor food and/or oxygen conditions.