Comparative study of dissolved inorganic carbon utilization and photosynthetic responses inNannochloris(Chlorophyceae) andNannochloropsis(Eustigmatophyceae) species

Abstract

Four species of marine microalgae with similar morphology and life cycle, namely Nannochloris atomus Butcher, Nannochloris maculata Butcher, Nannochloropsis gaditana Lubian, and Nannochloropsis oculata (Droop) Hibberd, have been examined with respect to their affinity for different sources of dissolved inorganic carbon. External carbonic anhydrase activity was not found in any of these species, but the cell affinity for dissolved inorganic carbon (DIC) in Nannochloris species was affected by the inhibitor acetazolamide at a concentration of 400 µM. Measurement of photosynthetic rates and CO2compensation points at different pH values showed that the Nannochloris species had a greater capacity for CO2rather than HCO3-utilization. In contrast, the observed rates of photosynthetic oxygen evolution in Nannochloropsis species were greater than could be accounted for by the theoretical rate of CO2supply from the spontaneous dehydration of bicarbonate in the external medium. This indicates that these algae were able to transport bicarbonate across the plasmalemma. Furthermore, the K0.5(DIC) value at acidic pH showed that Nannochloropsis oculata could also use CO2as an exogenous carbon source for photosynthesis. Although the species of marine phytoplankton used in this study possess similar morphological characteristics and life cycle, there exist many differences in the mode of inorganic carbon utilization between these microalgae.Key words: Nannochloris, Nannochloropsis, inorganic carbon utilization, bicarbonate transport, CO2compensation point, photosynthesis.