Abstract
For the first time, a simple and robust HPLC method has been developed for dissolution studies for cannabidiol (CBD) in hemp oil products. An isocratic elution of samples performed on SOLAS™ C18 150mm x 4.6mm, 5μm column with a mobile phase consisting of 75:25 acetonitrile-water v/v, delivered at a flow rate 1.5mL/minutes to variable wavelength detector using 214nm. An in-house validated assay test was executed for calculating the purity of hemp oil products and also for considering the dissolution medium to be used. For dissolution studies, equivalent of 5mg and/or 10mg of the active was introduced into 500mL of simulated gastric and intestinal fluids separately, and dissolution was performed at 50rpm using paddles for 180min. Dissolution profiles for hemp oil products purchased from the United States and Europe were compared. Additionally, dissolution testing was conducted to study the effect of percentage CBD release on increased agitation speed of 75 and 100rpm and also, on extended dissolution runtime of 240min.
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