Abstract
Spawn and bed production studies were carried out with isolate of Pleurotus cystidiosus having Genbankaccession number KY887023. P. cystidiosus took the minimum time for spawn run in paddy grains amended with one percent yeast extract (5.63 days), with thicker and fluffy mycelial growth and hence identified as the best amended substrate.Spraying of rubber wood sawdust with 2.5 per cent of 1 M Potassium Dihydrogen Phosphate (KH2PO4) resulted inproduction of the maximum BE (192.76 per cent) by Pleurotus sp. Multi locational trials with P. cystidiosus proved thespecies as the best oyster mushroom for low temperature areas of Kerala like Idukki and Wayanad
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