Comparative Study of Different Concentration of Trypan Blue for Staining of Capsule in Mature Cataract using RGB Scale in “Adobe Photoshop Image Reader”

Abstract

Satisfactory capsulorhexis is essential in modern small incision cataract surgery. Visualization of capsule in mature cataract is difficult so staining of capsule with dye is required. Trypan blue is most commonly used dye. Carcinogenic effect of trypan blue is proved in animal study. Aim of this study was to determine minimum concentration of trypan blue that stains the anterior capsule. Methods: The study was conducted in 50 patients with senile mature cataract. Capsular staining was done with different concentration of trypan blue (Group A 0.06%, Group B 0.03%, Group C 0.015%, Group D 0.0075% and Group E 0.00375%) to enhance visualization of capsule for rhexis in cataract surgery. Comparative study of blueness of dye with different concentration was done by RGB scale values in “Adobe Photoshop image reader” Software (Version-7). Results : Trypan blue stains the capsule satisfactorily in group A, B and C. In group D and E capsular staining was not satisfactory. Conclusion: Concentrations of 0.03% and 0.015% were as effective as 0.06% to stain the capsule. Concentration of 0.0075% and 0.00375% could not stain the capsule.