Abstract
This paper reports the efficacy of nonthermal plasma (NTP) as a biocidal agent to treat 304 stainless steel (SS304) covered with a diamond-like carbon (DLC) film contaminated with Candida albicans biofilms. The association of two techniques using electric plasma was used. The first was plasma-enhanced chemical vapor deposition (PECVD) used to deposit a DLC film on the SS304. The second was NTP used on the surface of the SS304 with and without the DLC film. The combination of the SS304 surface with the DLC film was demonstrated to be effective when using the DLC film as corrosion protection. Comparing the proliferation of Candida species on the DLC film and SS304 surface, it was possible to conclude that Candida species stays longer in the DLC film than in SS304. The reduction of colony numbers was visible after 5 min using plasma on both surfaces; in addition, 99% of Candida species were eliminated after 15 min. Three C. albicans microorganisms were used. Two were from samples of urine and tracheal secret...
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