Abstract
Genetic modification of mesenchymal stem cells (MSCs) with plasmid encoding the bone morphogenetic protein 2 gene (BMP2) is a crucial task in the development of gene therapy and tissue engineering technologies for bone regeneration. The low transfection efficiency of MSC cultures is a limitation for practical applications and makes it necessary to search for optimal protocols that ensure efficient gene delivery while maintaining sufficient cell viability. Comparison of two transfecting reagents (TurboFect and Polyethylenimine) shows that TurboFect is the most effective for MSCs transfection. A higher level of target gene BMP2 expression and osteogenic differentiation can be achieved using the TaqRFP-N-BMP2 plasmid compared with pcDNA3-BMP2.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
