Abstract
The incidence of nosocomial infections by fungi have increased in recent decades, and 80% are caused by Candida species. The aim of this study was to characterize Candida yeasts isolated from central venous catheter (CVC) and blood cultures of hospitalised patients. Approximately 65% of the infections result from the migration of microorganisms the microflora of the skin, from the insertion site of the catheter and are due to biofilm formation. The methodology employed was to identify CVC and blood culture samples using CHROMagar®, CANDIFAST® and the automated VITEK® 2 Compact. To assess biofilm formation, we used a Scanning Electron Microscopy EVO MA 10 (SEM). The yeasts were the second microorganism most prevalent (17%) on catheter tip and blood culture along with Klebsiella pneumoniae and Staphylococcus epidermidis. We investigated 10 samples and 73% were identified through automated methodology, being 26% of strains of Candida albicans, 13% C. parapsilosis and C. tropicalis, 6% de C. glabrata, C. pelliculosa and C. guilliermondii. When analyzing the percentage of agreement between the results obtained in the three methodologies used, 80% of the strains had 67% agreement. Using SEM technique all samples were positive for catheter biofilm formation, concluding that their presence may influence the catheter‐related candidemia and that there is no single ideal method for identifying these emerging fungi.
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