Comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria

Abstract

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO2 and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose.The experimental results compared the hydrogen production yield of 19 different pure strains and sludges: facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1.36mol H2/mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains.