Comparative Study of Bioactive Substances Extracted from Fresh and Dried Spirulina sp.

Abstract

The increase consumption of natural substances has brought about in increasing demand on biological source, including Spirulina sp. This Spirulina sp. has been recognized to provide some natural substances such as natural colorant, source of vitamins, protein and some minerals. Different material state of Spirulina sp. in the form of fresh and dried form may cause different quantity and quality of natural substances in microalgae which posses a wide range of change including nutritional and natural substances. The aims of the study was to investigate the bioactive compounds resulted from different form of Spirulian sp (fresh and dried) quantitatively. The materials used were fresh and dried Spirulina sp which was extracted by ethanol. Analysis were subjected for phytochemical screening, flavoniod, phenolic acid and antioxidant activity (DPPH scavenging activity). The results showed that total flavonoid of fresh and dried are: 25.6615±1.62 and 110.1356±12.5 quercetin/gr extract; phenolic acid: 2.117±0.99 and 6.92±0.03 GAE/gr extract; IC50: 33.0755, respectively. Fresh sample of Spirulina platensis shows better results in bioactive compounds quantitatively and qualitatively compared to that of dried sample, and drying process at 40-50oC does not give any significant different on nutritional quality of dried sample of Spirulina platensis compared to fresh samples.