Comparative study of antibacterial activity between &lt;i&gt;Cynodon dactylon&lt;/i&gt; crude and solid phase extraction extracts against selected bacterial pathogens

Syahriel Abdullah,Januarius Gobilik,Khim-Phin Chong

doi:10.3329/bjp.v9i4.20616

Abstract

Previous studies have shown Cynodon dactylon extracts possess broad spectrum of antibacterial activity against common-known Gram positive and negative bacteria. It is believed that specific extraction procedure can enhance the efficacy of extracting antibacterial compounds in plants, thus influence the biological activity of the extract. The present study compared the antibacterial activity of C. dactylon crude and Solid Phase Extraction (SPE) extracts against some common bacterial pathogens using disc diffusion and broth micro-dilution assays. This study also revealed the flush fraction of C. dactylon ethanol and ethyl acetate SPE extracts with greater antibacterial activity compared to the plant crude extracts. MIC tests confirmed the greatest antibacterial activity is from flush fraction of C. dactylon ethanol SPE extract with MIC values of 10.00-20.00 mgmL-1. Comparative study suggests extraction using SPE to obtain the plant extract can produce greater antibacterial activity in contrast to conventional method of extraction. Discover

Highlights

Previous studies shown C. dactylon killed some common bacterial pathogens including common nosocomial-caused infection pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa (Abdullah et al, 2012; Abdullah et al, 2013)
Our present study compared the antibacterial activity of C. dactylon crude and Solid Phase Extraction (SPE) flush fraction extracts against Bacillus cereus, Bacillus subtilis, E. coli, Klebsiella spp., P. aeruginosa, S. aureus, Streptococcus pyogenes and Streptococcus pneumonia using disc diffusion and broth micro-dilution bioassays
It is observed that the size of inhibition for the crude extracts is between 7.0 ± 0.0 mm to 10.0 ± 1.0 mm for ethanol extract and 7.0 ± 0.0 to 12.0 ± 1.0 mm for ethyl acetate extract

Summary

Introduction

Comparative study of antibacterial activity between Cynodon dactylon crude and solid phase extraction extracts against selected bacterial pathogens Previous studies shown C. dactylon killed some common bacterial pathogens including common nosocomial-caused infection pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa (Abdullah et al, 2012; Abdullah et al, 2013).

Results

Conclusion