Abstract
We investigated the influence of the transcription product, tRNA, on the transcriptional efficiency of its structural gene in a Xenopus laevis in vitro system. Addition of tRNA to the assays leads to a decreased transcriptional efficiency. While genes with high transcriptional efficiencies react strongly to the addition of bulk tRNA, those with low efficiencies react less markedly. One of the genes tested contains an unusual 5′ flanking sequence—a tRNA-pseudogene—leading to an oversized product in this system. The amount of this product is positively influenced when a low dose of tRNA is added, but the addition of a larger amount of tRNA decreases its transcriptional efficiency. We also present a comparative computer study of published eukaryotic tRNA gene data. In most tRNA genes, there is a box consisting of four purines between two pyrimidines in the 5′ flanking sequence. This box is surrounded by or is part of repeating four-nucletide sequences. In addition there is a slight homology of the 5′ flank with its following structural gene.
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