Abstract
1. 1. This study was undertaken to characterize the α-glucosidase present in blood and seminal plasma of two strains of pure-bred rams which are known as seasonal breeders. 2. 2. pH profiles and activity levels were investigated in blood and seminal plasma using a sensitive spectrophotometric assay with para-nitrophenyl-α- d-glucoside as substrate. 3. 3. According to their pH optimum, blood plasma and seminal plasma α-glucosidases were typically neutral and acid enzymes and significant differences were recorded in their physico-chemical properties, establishing the tissue specificity of the enzyme. 4. 4. Notwithstanding the tissue under study, the nature of the α-glucosidase activity was similar in both strains of pure-bred rams.
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