Abstract
Toxoplasma gondii caused a widely distributed protozoan disease called Toxoplasmosis. “The infective stage of T. gondii is capable of infecting variety of vertebrates including humans, domestic animals and wild felids are capable of serving as definitive hosts and T. gondii Oocyst are excreted in their feces”. “Toxoplasmosis is caused by ingestion T. gondii Oocyst from contaminated soil or water or foodstuff or by consuming T. gondii tissue cysts from infected hosts”. “The present study was designed to develop methods for detection of Oocyst from soil by nested PCR using Toxoplasma gene B1 primers”. The results in this study noted that the contamination was highest prevalence in gardens with T. gondii, schools and “backyards of homes” that is in Baghdad city (21.42%, 17.4%, 16.66%) respectively and in the Kut city is (6%, 5.5%, 4%) respectively. rural area infection with T. gondii was highest in the two cities Baghdad and Kut.
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