Abstract
Background: Tuberculosis remains a major public health problem worldwide. Nucleic acid amplification tests (NAATs) have been introduced to overcome conventional methods’ low sensitivity and diagnostic delays. These rapid tests such as the line probe assay (LPA) and XpertMTB/RIF assay can provide results within hours directly on specimens and thus enable prompt and appropriate treatment, decrease morbidity and mortality, and interrupt transmission. Objectives: The aim of this study was to evaluate the efficiency and reliability of MTBDRplus and XpertMTB/RIF assay for the detection of Mycobacterium tuberculosis complex (MTBC) in smear-positive and smear-negative pulmonary and extrapulmonary specimens in comparison to the gold standard VersaTREK liquid culture system. Methodology and Results: In this study, a total of 130 samples were obtained from tuberculous patients. The percentages of the MTBC detection in AFB-negative samples by Hain PCR was 0.0%,while, the percentages of the MTBC detection in AFB-positive (+1), (+2), (+3) and(+4) samples by Hain PCR were 0.0%,55.6%, 75% and 93.8%. The percentage of the MTBC detection in AFB-negative samples by GeneXpert PCR was higher than those which were detected by Hain PCR (30.4% vs 0.0%) with high significant difference. Mean while, the percentage of the MTBC detection in AFB-positive (+1) samples by GeneXpert PCR was higher than those which were detected by Hain PCR (100% vs 0.0%) with high significant difference. The percentage of the MTBC detection in total AFB-positive samples by GeneXpert PCR was higher than those which were detected by Hain PCR (90.5% vs 54.8%) with high significant difference. The sensitivity, specificity and accuracy of Gene Xpert PCR were 64.9%,0.0% and 56.9% while, they were40.4%,100% and 47.6% by Hain PCR. The degree of detection of MTBC by GeneXpertPCR was correlated with the results of AFB staining with high significant difference. Conclusion : our findings showed the superior sensitivity of the Xpert MTB/RIF compared to the MTBDRplus in the detection of MTBC. However, the molecular drug susceptibility testing results should always be confirmed by phenotypic methods.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
