Abstract

Cytotoxic and antimicrobial activities of ethyl acetate extract of mangrove plant Scyphiphora hydrophyllacea C. F. Gaertn— Associated fungiDian Handayani, Neny sandrawati, Rustini Ruslan, Oga Nestianda, Anzharni Fajrina, Trina Ekawati Tallei

Highlights

  • Polysaccharides, like mannans, are found as linear homopolysaccharides, heteropolysaccharides or branched form

  • Qualitative examination of the hydrolytic action of these fungi, revealed that all isolates were negative except P. chrysogenum exhibited the promising mannanase activity (80 U/mL)

  • The results indicated clearly to the effect of immobilization process in protect the enzyme activity to great extent in compared to the free form while, the free enzyme lost 75% of its original activity at 70 ̊C and the immobilized form kept 76.4 % of its activity at the same degree

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Summary

Introduction

Polysaccharides, like mannans, are found as linear homopolysaccharides, heteropolysaccharides or branched form. Mannans are one of the major components of hemicelluloses and in the yeast cell wall. Mannanases are enzymes which have a great impact in the application of industrial processes such as biobleaching of pulp, detergent industry, food and pharmaceutical productions e.g. mannooligosaccharides (MOS) (Mc Cleary et al, 1988), in various other industries. The composition of mannan is mainly influenced by the action of more than one enzyme. That degradation of heteromannan, like locust bean gum, can be achieved by fungi and bacteria using different microbial enzymes.

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