Comparative Study between Croton tiglium Seeds and Moringa oleifera Leaves Extracts, after Incorporating Silver Nanoparticles, on Murine Brains

Abstract

Croton tiglium seeds and Moringa oleifera leaves extracts are rich in phytoconstituents with the antioxidant efficiency which can be enhanced by incorporating silver nanoparticles (Ag-NPs). The present study was designed to compare the effect of C. tiglium seeds and M. oleifera leaves nano-extracts on brain tissues of murine models. During the current study, acetylcholine esterase (ACHE), β-amyloid (Aβ) content and inflammatory markers were measured in brain tissues. Moreover, native protein, lipoprotein and isoenzymes patterns were electrophoretically detected. Also, the interferon-gamma (INF-γ) receptor protein was studied by molecular dynamic simulation to evaluate the significant alterations on brain tissues. It was found that ACHE, Aβ contents and inflammatory markers increased in the C. tiglium nano-extract treated group at a dose of 6.5 ml/kg. Furthermore, it caused qualitative electrophoretic abnormalities represented by lowering similarity index (SI) values. Also, the resides range 119~127 represent the most reactive and flexible site in INF-γ receptor protein. On the other hand, it was shown that no significant differences were induced by silver M. oleifera nano-extract.