Abstract
Abstract— Genetic effects of UV‐A, UV‐B, UV‐C, and the combination of 8‐methoxypsoralen (8MOP) with UV‐A or visible light were studied in the haploid strain XV185–14C and diploid strain D5 of Saccharomyces cerevisiae. The induction of his+, lys+, and horn+ reverse mutations was measured in strain XV185–14C. In strain D5 we measured the induction of genetically altered colonies, particularly twin spot colonies arising from a mitotic crossing‐over. UV‐C and UV‐B induced point mutations at the three loci in the haploid strain and mitotic crossing‐over and other genetic alterations in the diploid strain. UV‐C was more mutagenic and recombinogenic than UV‐B. UV‐A or visible light alone did not induce genotoxic effects at the doses tested. However, UV‐A plus 8‐MOP produced lethal and mutagenic effects in the haploid strain XV185–14C, although mutagenic activity was less than that of UV‐B. Visible light plus 8‐MOP also induced genotoxic effects in strain XV185–14C. In the diploid strain D5, UV‐A plus 8‐MOP induced a higher frequency of genetic alterations than UV‐B at comparative doses. Visible light plus 8‐MOP was also genetically active in strain D5. The haploid strain was more sensitive to the lethal effects of UV‐C, UV‐B, UV‐A, and impure visible light plus 8‐MOP than the diploid strain.
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