Comparative studies on the isoenzymes of glutathione S-transferase of rat brain and other tissues

Abstract

1. 1. Six forms of glutathione S-transferase (GST) designated as GST 9.3, GST 7.5, GST 6.6, GST 6.1, GST 5.7 and GST 4.9 have been purified to homogeneity from rat brain. 2. 2. All GST isoenzymes of rat brain are apparent homodimers of one of the three type subunits, Ya, Yb, or Ye. More than 60% of total GST activity of rat brain is accounted for by the near neutral and anionic isoenzymes and about 55% of total brain GST activity is associated with the isoenzymes containing only the Yb type of subunits. In these respects brain GST isoenzymes differ from those of lung and liver. 3. 3. The Ya, Yb, and Yc type subunits of brain GST are immunologically similar to the corresponding subunits of liver and lung GST. The isoelectric points and kinetic properties of the Yb type subunit dimers in brain are strikingly different from those of the Yb type dimers present among liver GST isoenzymes indicating subtle differences between these subunits of brain and liver.