Abstract
1. 1. Sequence analyses of Bence-Jones proteins up to 15 amino acids from the N-terminus provide decision of subgroups. 2. 2. Investigation of primary structure of 3 Bence-Jones proteins, monomer and dimer of a kappa type, is limited at position 9–10 using DABITC reagent, whereas DABITC/PITC double coupling method allows sequencing up to 20–21 amino acids. 3. 3. Sequencing of tryptic peptides allows only determination of 6–9 amino acids. 4. 4. Microsequencing of tryptic peptide T13a shows the allotypic variant, inv b +, of Bence-Jones proteins TRA and GAN.
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